# The Options directive is both complicated and important. Please see
              # http://httpd.apache.org/docs/2.2/mod/core.html#options
              # for more information.
              # 
              Options Indexes FollowSymLinks

              #
              # AllowOverride controls what directives may be placed in .htaccess files.
              # It can be "All", "None", or any combination of the keywords:
              #   Options FileInfo AuthConfig Limit
              # 
              AllowOverride None

              #
              # Controls who can get stuff from this server.
              # 
              Order allow,deny 
              Allow from all

          </Directory>
          ......

   在Options Indexes FollowSymLinks在Indexes前面加上 - 符号。
        即： Options -Indexes FollowSymLinks
   【备注：在Indexes前，加 + 代表允许目录浏览；加 - 代表禁止目录浏览。】

    这样的话就属于整个Apache禁止目录浏览了。

    如果是在虚拟主机中，只要增加如下信息就行：
           <Directory "D:\test">
             Options -Indexes FollowSymLinks
             AllowOverride None